abstract：:The synthesis of galactooligosaccharides (GOS) by whole cells of Bifidobacterium bifidum NCIMB 41171 was investigated by developing a set of mathematical models. These were second order polynomial equations, which described responses related to the production of GOS constituents, the selectivity of lactose conversion ...

journal_title：Journal of biotechnology

authors： Osman A,Tzortzis G,Rastall RA,Charalampopoulos D

更新日期：2010-10-01 00:00:00

abstract：:Clostridium thermocellum encodes a xylanase gene (xynC) which is the major component of its cellulosome. XynC is a multidomain enzyme comprising of a substrate binding domain at the N-terminal followed by the catalytic domain and a dockerin domain. To study the influence of binding domain on activity, stability and ex...

journal_title：Journal of biotechnology

authors： Khan MI,Sajjad M,Ali I,Ahmad S,Akhtar MW

更新日期：2010-10-01 00:00:00

abstract：:Super-resolution fluorescence imaging methods based on reversible photoswitching of fluorophores with subsequent localization currently develop to promising tools for cellular imaging. Since most of these methods rely on the transfer of the majority of fluorophores to a non-fluorescent dark state and precise localizat...

journal_title：Journal of biotechnology

authors： van de Linde S,Wolter S,Heilemann M,Sauer M

更新日期：2010-09-15 00:00:00

abstract：:Corynebacterium glutamicum is one of the best studied organisms of the high G+C branch of Gram-positive bacteria and an emerging model system for the suborder Corynebacterineae. To gain insights into the regulatory gene composition and architecture of the transcriptional regulatory network of C. glutamicum, components...

journal_title：Journal of biotechnology

authors： Brinkrolf K,Schröder J,Pühler A,Tauch A

更新日期：2010-09-01 00:00:00

abstract：:The Pichia anomala gene PPHY, which codes for a cell-bound phytase, was isolated from genomic DNA by PCR, using oligonucleotide sequences derived from the N-terminal region of the purified phytase protein (Pphyp) and a degenerate primer derived from conserved sequences of yeast and fungal phytases as primers. The gene...

journal_title：Journal of biotechnology

authors： Kaur P,Singh B,Böer E,Straube N,Piontek M,Satyanarayana T,Kunze G

更新日期：2010-08-20 00:00:00

abstract：:Endo beta-1,4-mannanases (beta-mannanases, EC 3.2.1.78), belonging to CAZy GH5 and GH26 families, catalyze the hydrolysis of structurally different mannans. In this study, the mannanase encoding gene of Aspergillus aculeatus VN was expressed in Aspergillus niger D15#26 using pAN 52-4 vector, under the control of PgpdA...

journal_title：Journal of biotechnology

authors： Pham TA,Berrin JG,Record E,To KA,Sigoillot JC

更新日期：2010-08-02 00:00:00

abstract：:The successful transfer of human embryonic stem cell (hESC) technology and cellular products into clinical and industrial applications needs to address issues of automation, standardization and the generation of relevant cell numbers of high quality. In this study, we combined microcarrier technology and controlled st...

journal_title：Journal of biotechnology

authors： Serra M,Brito C,Sousa MF,Jensen J,Tostões R,Clemente J,Strehl R,Hyllner J,Carrondo MJ,Alves PM

更新日期：2010-08-02 00:00:00

abstract：:Six thermophilic enzymes from Thermus thermophilus were used to construct an 'artificial bio-synthetic pathway' for the production of 2-deoxyribose 5-phosphate from fructose. By a simple operation using six recombinant Escherichia coli strains producing the thermophilic enzymes, respectively, fructose was converted to...

journal_title：Journal of biotechnology

authors： Honda K,Maya S,Omasa T,Hirota R,Kuroda A,Ohtake H

更新日期：2010-08-02 00:00:00

abstract：:Dynamic miniaturized human multi-micro-organ bioreactor systems are envisaged as a possible solution for the embarrassing gap of predictive substance testing prior to human exposure. A rational approach was applied to simulate and design dynamic long-term cultures of the smallest possible functional human organ units,...

journal_title：Journal of biotechnology

authors： Sonntag F,Schilling N,Mader K,Gruchow M,Klotzbach U,Lindner G,Horland R,Wagner I,Lauster R,Howitz S,Hoffmann S,Marx U

更新日期：2010-07-01 00:00:00

abstract：:A stably transformed insect cell expression system is superior to a baculovirus expression system, since the expression system is sustained and there is no cell lysis, but the isolation of cell lines producing recombinant proteins is time-consuming and laborious. In this study, we developed a technique for the rapid i...

journal_title：Journal of biotechnology

authors： Kato T,Yoshizuka K,Park EY

更新日期：2010-05-17 00:00:00

abstract：:Software sensors for monitoring and on-line estimation of critical bioprocess variables have mainly been used with standard bioreactor sensors, such as electrodes and gas analyzers, where algorithms in the software model have generated the desired state variables. In this article we propose that other on-line instrume...

journal_title：Journal of biotechnology

authors： Warth B,Rajkai G,Mandenius CF

更新日期：2010-05-03 00:00:00

abstract：:This study examined the kinetics and substrate selectivity of a GH11 Bacillus subtilis XynA xylanase (BsX) sensitive to inhibition by TAXI and an engineered variant, which is much less inhibited by TAXI (BsX(mut)). The main purpose of the work was to elucidate any influence of the structural point mutations on the kin...

journal_title：Journal of biotechnology

authors： Rasmussen LE,Sørensen JF,Meyer AS

更新日期：2010-04-15 00:00:00

abstract：:Human prolactin (hPRL) is a 199 aminoacid protein hormone with a wide spectrum of biological activities which is best known for its stimulation of lactation and development of mammary gland. This protein contains only one potential asparagine-linked glycosylation site, which is partially (10-30%) occupied when the pro...

journal_title：Journal of biotechnology

authors： Heller SR,Rodrigues Goulart H,Arthuso FS,Oliveira TL,Bartolini P,Soares CR

更新日期：2010-02-15 00:00:00

abstract：:A continuous enzymatic process for the production of the prebiotic disaccharide lactulose through transgalactosylation was developed using free and immobilized beta-glycosidase from Pyrococcus furiosus. The hyperthermostable beta-glycosidase (CelB) was immobilized onto an anion-exchange resin (Amberlite IRA-93) or ont...

journal_title：Journal of biotechnology

authors： Mayer J,Kranz B,Fischer L

更新日期：2010-02-15 00:00:00

abstract：:Clostridium cellulovorans produces large extracellular enzyme complex, called cellulosomes. The diversity of the cellulosomal enzymes, which are secreted by C. cellulovorans that has been cultured on different carbon sources, such as Avicel, xylan, AXP (Avicel-xylan-pectin, 3:1:1) and cellobiose, was explored by two-d...

journal_title：Journal of biotechnology

authors： Cho W,Jeon SD,Shim HJ,Doi RH,Han SO

更新日期：2010-02-01 00:00:00

abstract：:The present work describes the development of a novel direct screening method, assayed in 96-well format, for evaluation of enzymatic alkyl glycoside production in a hexanol-water two-phase system. Alkyl glycosides are surfactants with a range of applications and with good biodegradability and low toxicity. Enzymatic ...

journal_title：Journal of biotechnology

authors： Gräber M,Andersson M,Rundbäck F,Pozzo T,Karlsson EN,Adlercreutz P

更新日期：2010-01-15 00:00:00

abstract：:Myostatin (MSTN), a member of transforming growth factor-beta superfamily is a negative regulator of the skeletal muscle growth. It suppresses the proliferation and differentiation of myoblast cells. Dysfunction of MSTN gene either by natural mutation or induced through genetic manipulation (knockout or knockdown) has...

journal_title：Journal of biotechnology

authors： Jain H,Singh S,Kadam M,Sarkhel BC

更新日期：2010-01-15 00:00:00

abstract：:Oral infection of insect larvae with baculovirus is an advantageous methodology for producing high levels of recombinant proteins and for achieving plague control. However, many recombinant baculoviruses express a foreign protein in lieu of the polyhedrin and hence do not form occlusion bodies (occ-), resulting in ext...

journal_title：Journal of biotechnology

authors： López MG,Alfonso V,Carrillo E,Taboga O

更新日期：2010-01-15 00:00:00

abstract：:To develop a receptor-mediated intracellular delivery system that can transport therapeutic proteins to specific tumor cells, folate-poly(ethylene glycol)-grafted-trimethylchitosan (folate-PEG-g-TMC) was synthesized. Nano-scaled spherical polyelectrolyte complexes between the folate-PEG-g-TMC and fluorescein isothiocy...

journal_title：Journal of biotechnology

authors： Zheng Y,Song X,Darby M,Liang Y,He L,Cai Z,Chen Q,Bi Y,Yang X,Xu J,Li Y,Sun Y,Lee RJ,Hou S

更新日期：2010-01-01 00:00:00

abstract：:Ubiquitination is one of the most significant posttranslational modifications (PTMs). To evaluate the ability of an insect cell-free protein synthesis system to carry out ubiquitin (Ub) conjugation to in vitro translated proteins, poly-Ub chain formation was studied in an insect cell-free protein synthesis system. Pol...

journal_title：Journal of biotechnology

authors： Suzuki T,Ezure T,Ando E,Nishimura O,Utsumi T,Tsunasawa S

更新日期：2010-01-01 00:00:00

abstract：:Overcoming the effects of hydrolysate toxicity towards ethanologens is a key technical barrier in the biochemical conversion process for biomass feedstocks to ethanol. Despite its importance, the complexity of the hydrolysate toxicity phenomena and the lack of systematic studies, analysis and tools surrounding this is...

journal_title：Journal of biotechnology

authors： Franden MA,Pienkos PT,Zhang M

更新日期：2009-12-01 00:00:00

abstract：:Batch bioreactor cultivations using Saccharomyces cerevisiae at high (190-305 gl(-1) glucose) or low (21-25 gl(-1) glucose) gravity conditions were monitored on-line using multi-wavelength fluorescence (MWF) and standard monitoring sensors. Partial least squares models were calibrated for the prediction of cell dry we...

journal_title：Journal of biotechnology

authors： Odman P,Johansen CL,Olsson L,Gernaey KV,Lantz AE

更新日期：2009-10-26 00:00:00

abstract：:Based on the prototype experiment of treating herb wastewater by Up flow Anaerobic Sludge Bed and Anaerobic Filter reactor (UASBAF), an artificial neural network (ANN) model which adopts a back propagation algorithm with momentum and adaptive learning rate was established. And the effect of each parameter to the perfo...

journal_title：Journal of biotechnology

authors： Shi Y,Zhao XT,Zhang YM,Ren NQ

更新日期：2009-10-12 00:00:00

abstract：:For biotechnological production of coenzyme Q(10) (CoQ(10)) in recombinant Escherichia coli, three genetic manipulations were performed: heterologous expression of decaprenyl diphosphate synthase (Dps) from Agrobacterium tumefaciens, deletion of endogenous octaprenyl diphosphate synthase (IspB), and overexpression of ...

journal_title：Journal of biotechnology

authors： Choi JH,Ryu YW,Park YC,Seo JH

更新日期：2009-10-12 00:00:00

abstract：:Corynebacterium glutamicum is an industrially important producer of amino acids and an emerging model system for the Corynebacterineae. The glxR gene of C. glutamicum ATCC 13032 encodes a DNA binding transcription factor of the Crp-Fnr protein family. Available data indicated a prominent role of GlxR in the transcript...

journal_title：Journal of biotechnology

authors： Kohl TA,Tauch A

更新日期：2009-09-25 00:00:00

abstract：:Cannabis sativa L. plants produce a diverse array of secondary metabolites. Cannabis cell cultures were treated with biotic and abiotic elicitors to evaluate their effect on secondary metabolism. Metabolic profiles analysed by (1)H NMR spectroscopy and principal component analysis (PCA) showed variations in some of th...

journal_title：Journal of biotechnology

authors： Flores-Sanchez IJ,Pec J,Fei J,Choi YH,Dusek J,Verpoorte R

更新日期：2009-08-20 00:00:00

abstract：:Mining sequence data is increasingly important for biocatalysis research. However, when relying on sequence data alone, prediction of the reaction catalyzed by a specific protein sequence is often elusive, and substrate specificity is far from trivial. The present study demonstrated an approach of combining sequence d...

journal_title：Journal of biotechnology

authors： Seffernick JL,Samanta SK,Louie TM,Wackett LP,Subramanian M

更新日期：2009-08-10 00:00:00

abstract：:Recombinant human granulocyte colony stimulating factor (rhG-CSF) and its PEGylated product "mono-PEG20-GCSF" have already been widely used for treatment of all kinds of neutropenia. However, the high required dosage of mono-PEG20-GCSF made it relatively expensive in clinical use. We postulated that an N-terminal site...

journal_title：Journal of biotechnology

authors： Zhai Y,Zhao Y,Lei J,Su Z,Ma G

更新日期：2009-07-15 00:00:00

abstract：:The biocatalytic efficiency of recombinant Corynebacterium glutamicum expressing the chnB gene encoding cyclohexanone monooxygenase (CHMO) of Acinetobacter calcoaceticus NCIMB 9871 was investigated. Optimization of an expression system and induction conditions enabled the recombinant biocatalyst to produce CHMO to a s...

journal_title：Journal of biotechnology

authors： Doo EH,Lee WH,Seo HS,Seo JH,Park JB

更新日期：2009-06-15 00:00:00

abstract：:Glutamate dehydrogenase is a central enzyme connecting nitrogen and carbon metabolism via its precursors ammonium and oxoglutarate and its product glutamate. In Corynebacterium glutamicum glutamate dehydrogenase is especially important, since it is a key enzyme for the biotechnological production of the flavour enhanc...

journal_title：Journal of biotechnology

authors： Hänssler E,Müller T,Palumbo K,Patek M,Brocker M,Krämer R,Burkovski A

更新日期：2009-06-15 00:00:00

abstract：:In spite of high prices for fossil raw materials the production of biomass-based products is rarely economically successful today. Depending on the location feedstock prices are currently so high that products from renewable resources are not marketable when produced in existing process chains. Apart from the higher f...

journal_title：Journal of biotechnology

authors： Lyko H,Deerberg G,Weidner E

更新日期：2009-06-01 00:00:00

abstract：:Hepatitis B virus, a member of the hepadnavirus family, causes the acute and chronic diseases of the human liver. The S domain of hepatitis B virus surface antigen (sHBsAg) was expressed under the control of the galactose-inducible GAL1 promoter in recombinant Saccharomyces cerevisiae. Batch fermentation of S. cerevis...

journal_title：Journal of biotechnology

authors： Kim EJ,Park YK,Lim HK,Park YC,Seo JH

更新日期：2009-05-20 00:00:00

abstract：:Plasmid DNA purification strategies are often based on chromatographic processes, which often suffer from low dynamic binding capacities and poor productivity. The limitations of conventional chromatography media for adsorption of pDNA led us to the investigation of irregular polymeric microparticles with quaternary a...

journal_title：Journal of biotechnology

authors： Paril C,Horner D,Ganja R,Jungbauer A

更新日期：2009-04-20 00:00:00

abstract：:The application of artificial chaperones in biotechnology has been inspired by the mechanism of molecular chaperones like GroEL/GroES. It involves addition of a capturing detergent during dilution of the chaotropic reagent, that prevents protein aggregation, and finally, addition of a oligosaccharide that removes the ...

journal_title：Journal of biotechnology

authors： Laslo AC,Ganea E,Obinger C

更新日期：2009-03-25 00:00:00

abstract：:An enzyme-responsive artificial chaperone system which employs an amphiphilic amylose primer (dodecyl maltopentaose, C12-MP) as a surfactant and phosphorylase b was designed to enable protein refolding. Effective refolding of carbonic anhydrase B after both heat denaturation (70 degrees C for 10min) and guanidine hydr...

journal_title：Journal of biotechnology

authors： Morimoto N,Ogino N,Narita T,Akiyoshi K

更新日期：2009-03-25 00:00:00

abstract：:The effects of a deletion of the amtR gene, encoding the master regulator of nitrogen control in Corynebacterium glutamicum, were investigated by metabolome and transcriptome analyses. Compared to the wild type, different metabolite patterns were observed in respect to glycolysis, pentose phosphate pathway, citric aci...

journal_title：Journal of biotechnology

authors： Buchinger S,Strösser J,Rehm N,Hänssler E,Hans S,Bathe B,Schomburg D,Krämer R,Burkovski A

更新日期：2009-03-10 00:00:00

abstract：:Microarray analysis has become a popular and routine method in functional genomics. It is typical for such experiments to involve a small number of replicates, which causes unreliable estimates of the sample variance. Microarrays have fostered the development of new statistical methods to analyze data resulting from e...

journal_title：Journal of biotechnology

authors： Dondrup M,Hüser AT,Mertens D,Goesmann A

更新日期：2009-03-10 00:00:00

abstract：:New morphological aspects of Penicillium chrysogenum were found during physiological characterisation of two NADPH-dependent glutamate dehydrogenase mutant strains. A morphological characterisation of the previously constructed strains, together with the two beta-lactam producing industrial recipient strains, was cond...

journal_title：Journal of biotechnology

authors： Thykaer J,Rueksomtawin K,Noorman H,Nielsen J

更新日期：2009-02-23 00:00:00

abstract：:Transgenic hairy root (HR) systems constitute an interesting alternative to improve the efficiency of phytoremediation process. Since peroxidases (Px) have been associated with phenolic compounds removal, in the present work, transgenic tobacco HR, which expressed basic Px genes from tomato (tpx1 and tpx2), were estab...

journal_title：Journal of biotechnology

authors： Sosa Alderete LG,Talano MA,Ibáñez SG,Purro S,Agostini E,Milrad SR,Medina MI

更新日期：2009-02-23 00:00:00

abstract：:Late-embryogenesis abundant (LEA) proteins are a family of hydrophilic proteins that form an integral part of desiccation tolerance of seeds. LEA proteins have been also postulated to play a protective role under different abiotic stresses. Their role in abiotic stress tolerance has been well documented for Group 1, 2...

journal_title：Journal of biotechnology

authors： Dalal M,Tayal D,Chinnusamy V,Bansal KC

更新日期：2009-01-15 00:00:00